To inoculate mushroom spores for the first time, work inside a still air box, flame-sterilise your needle, and shake the syringe for 30 seconds. Inject 0.5–1ml per port into a pre-sterilised PF Tek Kit, needle at 45 degrees. Using a grain bag instead? Use roughly 3ml — too much moisture causes problems later, and one syringe already holds tens of thousands of spores. Seal any holes with micropore tape, then label everything with species and date. Keep it warm and dark at 18–24 … read more